منابع مشابه
Purification of dystrophin from skeletal muscle.
Dystrophin was purified from rabbit skeletal muscle by alkaline dissociation of dystrophin-glycoprotein complex which was first prepared by derivatized lectin chromatography. Dystrophin-glycoprotein complex was isolated from digitonin-solubilized rabbit skeletal muscle membranes by a novel two-step method involving succinylated wheat germ agglutinin (sWGA) chromatography and DEAE-cellulose ion ...
متن کاملProperties of Porcine Skeletal Muscle Cytosol
Thyroid hormone binding properties were evaluated in eytosol prepared from semitendinosus muscle of l-d, 3-wk and 5-too-old Yorkshire-Duroc crossbred pigs. Optimum thyroxine (T4) binding was obtained with 35 ug cytosolic protein in a solution containing 50 mM Tris-HCl buffer, pH 7.4, and 25 mM KC1 and 10 mM MgCI2. The half-time of maximum T4 binding was approximately 10 min. Protease digestion ...
متن کاملCharacterisation of dystrophin during development of human skeletal muscle.
Dystrophin, the 427 x 10(3) Mr product of the Duchenne muscular dystrophy (DMD) gene, was studied in human foetal skeletal muscle from 9 to 26 weeks of gestation. Dystrophin could be detected from at least 9 weeks of gestation at the sarcolemmal membrane of most myotubes, though there was differential staining with antibodies raised to various regions of the protein. Dystrophin immunostaining i...
متن کاملDystrophin insufficiency causes selective muscle histopathology and loss of dystrophin-glycoprotein complex assembly in pig skeletal muscle.
The purpose of this investigation was to determine the extent to which dystrophin insufficiency caused histomorphological changes in a novel pig model of Becker muscular dystrophy. In our procedures, we used a combination of biochemical approaches, including quantitative PCR and Western blots, along with a histological analysis using standard and immunohistological measures. We found that 8-wk-...
متن کاملIntegrative Analysis of Porcine microRNAome during Skeletal Muscle Development
Pig is an important agricultural animal for meat production and provides a valuable model for many human diseases. Functional studies have demonstrated that microRNAs (miRNAs) play critical roles in almost all aspects of skeletal muscle development and disease pathogenesis. To investigate the miRNAs involved in regulating different periods of skeletal muscle development, we herein performed a c...
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ژورنال
عنوان ژورنال: Proceedings of the Japan Academy, Series B
سال: 1990
ISSN: 0386-2208,1349-2896
DOI: 10.2183/pjab.66.145